Application

Immunoprecipitation Kit (Protein A) has been used for the immunoprecipitation of proteins from cellular extracts with Protein A Agarose.

General description

Kit contains all reagents necessary for cell lysis, solubilization, stabilization, and immunopurification of proteins.

Immunoprecipitation is a widely used method for the analysis of target antigens in complex mixture of proteins. The protein of interest can be concentrated and immunoaffinity -purified in one step on an analytical scale via a specific antibody. Often, immunoprecipitated proteins are functionally fully active and can be further analyzed with respect to enzymatic activity, interactions, modifications and structure.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Packaging

1 kit containing 5 components.

Preparation Note

Working solution: Lysis buffer/wash buffer 1The kit contains reagents for 125 ml of lysis buffer/wash buffer 1. Prepare at least a minimal volume of 25 ml, sufficient for four immunoprecipitations.To prepare 25 ml of lysis buffer/wash buffer 1 mix 5 ml core buffer, 3.75 ml NaCl, 2.5 ml detergent mix and 1 cOmplete tablet. Add water to a final volume of 25 ml.Solution is stable at 2 to 8 °C for 24 hours. When stored in aliquots at -15 to -25 °C, the solution is stable for at least four weeks. Mix thoroughly after thawing.Wash buffer 2The kit contains reagents for 50 ml of wash buffer 2. 2 ml of this buffer is required for one immunoprecipitation.To prepare 50 ml of wash buffer 2 mix 10 ml core buffer, 25 ml NaCl and 0.5 ml detergent mix. Add water to a final volume of 50 ml.Solution is stable at 2 to 8 °C. For longer periods, store aliquots at -15 to -25 °C. Mix thoroughly after thawing.Wash buffer 3The kit contains reagents for 25 ml of wash buffer 3. 1 ml of this buffer is required for one immunoprecipitation.To prepare 25 ml mix 1 ml core buffer and 0.25 ml detergent mix. Add water to a final volume of 25 ml.Solution is stable at 2 to 8 °C. For longer periods, store aliquots at -15 to -25 °C. Mix thoroughly after thawing.